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Always speak with the facility manager if your project requires a deviation from the guidelines outlined below.
Required: UCSC Bloodborne pathogen training.
See the IBSC facility manger to arrange a tour and overview of the core regulations.
Before using any of the biohoods, spray 70% ethanol on the inside surfaces and wipe down all items going into the hood. Pay special attention to bottles coming from the water baths, since these are the biggest source of contamination.
Each time you are finished in the area, please do the following:
Note: Environmental Health & Safety no longer recommends using the UV lights in biohoods. Seethe IBSC facility manager for details.
Ask for assistance before operating the centrifuges in both labs. They are different and require specific commands.
For ESC projects, you will be assigned space in one of eight incubators, depending on your sample source. One Hypoxia unit is available for low O2 studies or other controlled conditions.
Separate refrigerator space is available for hESC and mESC researcher. Please see the IBSC facility manager for assigned space if your lab does not already have one.
Weekly use for the SSCF facility in Sinsheimer is $45.00/per project. A project can be up to 3 researchers. New users will be charged a one-time $200 fee for training and facility orientation. Currently, we are not offering -80 or Liq. Nitrogen storage.