Flow Cytometry Facility


Cytometry Facility Resources

The IBSC Flow Cytometry Facility offers multicolor cell analysis by a BD  LSRII cell analyzer and single-cell sorting by a BD Biosciences FACS Aria cell sorter. We will work with you to design your experiment and develop your protocol. We encourage users to become self-operators if they expect to be use the cytometry facility regularly, but we do offer fully assisted cell sorting and analysis by appointment.

The facility is located on the 4th floor of the Biomedical Sciences Building. It is available for use by any interested researcher for stem cell research as well as other life sciences research. The cost to use the LSR or ARIA varies based on whether you are within or outside UCSC, and non-UCSC nonprofits receive a special rate.

This core facility is supported by the California Institute for Regenerative Medicine (CIRM) and the California Institute for Quantitative Biosciences (QB3).

New Users

If you are new to cytometry and need help setting up an experimental protocol or design, please contact the IBSC facility manager. After you turn in the IBSC safety questionnaire, we will schedule a time with you to discuss your project. We recommend that researchers do an initial analysis experiment before trying to do a serious sort. This will allow for some familiarity with the BD DIVA software and give you a general feeling for cell numbers, concentrations, etc.

Biohazard Procedures (BSL2)

All users must have an approved BUA on file and must fill out the IBSC safety questionnaire before doing any project in the facility.

Operator Support

Our normal staff hours are 7:00-3:30 pm.

We offer operator support, by appointment, for people who need to schedule their FACS and LSR times outside the normal staff hours. We cannot guarantee that every request can be accommodated, particularly on short notice. To request an operator appointment, please contact the IBSC facility manager. Include information about the instrument and times that would work for you, and include a good telephone contact number.

Acknowledgements in publications

In all publications of research conducted in the Sinsheimer cell culture lab, and research that used the FACSAria, please acknowledge the CIRM Shared Stem Cell Facility grant to UCSC (CL1-00506). 

In all publications of research conducted on the Biomed 4th floor (including the cell culture and virus room), in the vivarium, or using the LSR II cell analyzer, please acknowledge the CIRM Major Facility Award to UCSC (FA1-00617).