Research Faculty

UCSC faculty members involved in stem cell research span six departments in the Baskin School of Engineering and the Divisions of Physical & Biological Sciences, Humanities, and Social Sciences.

Their research takes several approaches: studies in key model organisms, such as the mouse, fruit fly, and worm, using genetics and molecular biology; computational approaches for understanding gene regulatory networks, expression patterns, and alternative splicing; bioinformatics approaches that integrate and display all large-scale data sets collected from stem cell research; and examination of the social and ethical issues surrounding stem cell research.

Individual projects focus on a number of biological systems, including germ line and nervous system development, epigenetic mechanisms of gene regulation, blood cell differentiation, mammalian embryonic cell fates, wound healing, and the immune system.

Molecular, Cell, & Developmental Biology faculty

Manny Ares
Studying post-transcriptional control of gene expression in a variety of cell types, in particular systems-level regulation mediated by sequestration and competition phenomena.

Susan Carpenter
The Carpenter lab focuses on understanding the complex molecular mechanisms that drive inflammation in the body. Recent evidence shows that a group of RNA molecules known as long noncoding RNAs (lncRNAs) plays important roles in diverse biological functions, including the inflammatory response. They are studying lncRNAs to gain an understanding of their function within cells of the immune system, which could lead to insight into human disease and perhaps novel targets for therapeutic intervention for inflammatory conditions

Bin Chen
The Chen lab studies development of the cerebral cortex in the mammalian brain focusing on three related questions. 1) What is the lineage relationship between cortical neural stem cells and different types of cortical excitatory neurons and glial cell types? 2) What mechanisms regulate the lineage progression of neural stem cells? 3) What molecular mechanisms direct the specification of different subtypes of cortical excitatory neurons from multipoint neural stem cells? Dr. Chen uses a combination of approaches, including mouse genetics, neural anatomy, ChIP-seq and genomics, and electrophysiology.

David Feldheim
Dr. Feldheim researches how retinal ganglion cells (RGC), the neurons that project information from the eye to the brain, develop during embryogenesis and are maintained in adulthood. He uses a combination of mouse molecular genetics, anatomical tracing, and neural activity recordings to determine the genes that are required for RGC health and function. His research provides insights into mechanisms that determine how visual circuits develop, as well as the mechanisms of neurodegenerative diseases, such as glaucoma, in which RGCs die.

Lindsay Hinck
Dr. Hinck uses the breast as a model system to study how extracellular factors and the niche regulate the balance between stem/progenitor cell expansion, renewal and differentiation. Recently, her lab has focused on determining how alveolar progenitor cells generate the millions of differentiated cells required for every pregnancy and estrus cycle. Elucidating the pathways governing these events is of great interest. This research addresses the public health concern of lactation insufficiency—a significant challenge  for women’s and children’s health worldwide. Hinck's studies are defining a targetable pathway to enhance milk production.

Jeremy Sanford
Post-transcriptional gene regulation governs the fate and function of virtually every human gene product. Dr. Sanford's goal is to determine the underlying molecular basis for RNA-targeted regulatory mechanisms. His laboratory uses stem cells as a model to discover the biological functions and mRNA targets of RNA binding proteins (RBPs). Sanford's research program focuses on understanding how the processes of alternative pre-mRNA splicing and translational control contribute to the gene regulatory programs that drive stem cell differentiation. 

Upasna Sharma
Dr. Sharma’s goal is to elucidate the mechanism of intergenerational epigenetic inheritance by examining how environmental conditions modulate specific epigenetic marks in germ cells and how those marks influence development of offspring. The possibility that environment can influence phenotypes in descendants has tremendous implications for basic biology and public health and policy. To elucidate the mechanism of intergenerational epigenetic inheritance, Dr. Sharma's lab examines three key steps: 1) how epigenetic information signals are generated in gametes, 2) how those signals are influenced by environment, and 3) how those signals influence early embryonic gene expression and development. To address these questions, Dr. Sharma uses a unique and powerful combination of molecular, genetic, reproductive, and genomic approaches in the mouse.

Susan Strome
Dr. Strome's goal is to understand how epigenetic information is transmitted across generations and during development, including its importance in the normal development of germ cells in the offspring. Epigenetic mechanisms enable gene expression and development to be regulated not only by DNA sequence, but also by how DNA is packaged into chromatin. In C. elegans, a set of histone-modifying enzymes enables the parental chromosomes inherited by embryos to transmit an epigenetic “memory of the germline” from parent germ cells to the primordial germ cells (PGCs) in offspring.  Germ cells that do not inherit that memory die. Strome's lab uses this as a powerful model system to study fundamental transgenerational epigenetic mechanisms. 

Bill Sullivan
Dr. Sullivan studies the role of endocytic vesicle trafficking in regulating stem cell differentiation and self-renewal. His lab takes advantage of the well-studied neuroblast stem cell model of the Drosophila third instar larva. In addition to being amenable to sophisticated molecular genetic techniques, fixed and live fluorescent analysis can be performed readily in this system. Specifically, Sullivan focuses on the role of Rab11, a key component of the recycling endosome, and its effectors on stem cell self- renewal. Over-expression of Nuf, a conserved Rab11 effector, disrupts stem cell self-renewal. Future studies include investigating the mechanisms underlying endosome segregation in stem cell divisions and the role of endosomal components in mediating stem cell self renewal.

John Tamkun
Using a combination of genetic, biochemical, and molecular approaches to study chromatin-remodeling complexes, including their roles in transcriptional regulation, structural maintenance, post-translational modifications, and cell fate specification.

Olena Morozova Vaske
Pediatric cancers are disorders of normal development and differentiation. The Vaske lab studies these disorders using a combination of bioinformatic, genomic and molecular biology approaches.

Zhu Wang
One of Dr. Wang's main goals is to dissect the internal and extrinsic signaling pathways that regulate the stem cell plasticity of prostate basal cells. During prostate organogenesis, epithelial basal cells behave as stem cells to produce luminal cells and neuroendocrine cells. This capacity is preserved, but restricted, in the adult organ, and is only reactivated during prostate epithelium regeneration after luminal layer damage. To understand the stem cell plasticity of basal cells, Wang's lab employs multiple approaches, including genetic lineage tracing, organoid culture, CRISPR genome-editing, and single cell RNA-seq to 1) investigate the signaling pathways that regulate basal-to-luminal differentiation, 2) characterize stromal niche cell types and markers, and 3) identify gene regulatory network within basal cells. 

Martha Zúñiga
Understanding the role of epithelial cell differentiation in epidermal wound healing and in cutaneous immune responses. Characterizing the development and homeostatic maintenance of regulatory T cells.

Yi Zuo
Dr. Zuo's lab studies glia-neuron interaction, structural and functional plasticity during development, learning, and pathologies. The lab is particularly interested in the neural circuit change in developmental psychiatric disorders.

Biomolecular engineering faculty

Camilla Forsberg
Dr. Forsberg's mission is to determine how hematopoietic stem cells (HSCs) achieve homeostasis in blood and immune systems throughout life. Using the mouse as a model, her lab investigates blood cell production and specification of hematopoietic cells in the very early embryo, during adulthood, and in aging. The Forsberg lab dedicates substantial effort towards understanding the epigenetic inheritance of properties in ontogeny and upon differentiation. Important goals are to determine how maternal immune challenges during pregnancy affect the development and function of fetal HSCs and their descendant blood and immune cells, and how exposure to immune insults during perinatal life affects life-long health and susceptibility to disorders later in life. 

David Haussler - bioinformatics
The Haussler lab combines mathematics, computer science, and molecular biology to study human development and evolution. The bioinformatics group develops new statistical and algorithmic methods to explore the molecular function and evolution of the human genome, integrating cross-species comparative and high-throughput genomics data to study gene structure, function, and regulation. 

David Haussler - wet lab (Sofie Salama)
The “wet lab” explores and validates predictions generated from computational genomic research about the evolution and function of human genes. The lab uses embryonic and induced pluripotent stem cells to investigate neurodevelopment and cancer development from a functional and evolutionary perspective. Research project areas include genome evolution, comparative genomics, alternative splicing, and functional genomics.  

Daniel Kim
Dr. Kim focuses on determining the molecular mechanism by which RAS signaling regulates the noncoding transcriptome during human embryonic development. Dr. Kim use genomic, single cell, and genome engineering approaches to decode the functions of RAS-regulated noncoding RNAs in human pluripotent stem cells. His lab also investigates the potential roles of RAS-regulated noncoding RNAs that are released in extracellular vesicles in the pluripotent state. Deepening our understanding of how this fundamental signaling pathway regulates the noncoding transcriptome will provide novel insights into pluripotent stem cell biology.  

Ali Shariati
How stem cells decide to choose between two conflicting fates, division vs. differentiation, is an unsolved mystery of stem cell biology. The overarching goal of the Shariati lab is to determine the mechanisms that link cell division to cellular differentiation in stem cells. The lab combines emerging genome-editing technologies with single cell imaging to determine regulatory principles of cell fate decisions in pluripotent stem cells. 

Josh Stuart
The Stuart lab uses data-driven approaches to identify and characterize genetic networks, investigate how they have evolved, and then use them to simulate and predict cellular behavior. The lab’s approach is to design computational models and algorithms that integrate high-throughput molecular biology datasets (genomic, epigenomic, and functional genomic) to predict cellular- and organism-level phenotypes. Dr. Stuart’s group created the metadata standards by which all data in UCSC Stem Cell Hub (SCHub) was annotated. The standard defined the minimal information about a stem cell experiment (MISCE) and has been used for the length of the project to create coherent descriptions across labs. The Computational Genomics Laboratory assists in core informatics processing of data and in downstream analysis of gene expression and epigenomics to identify cell states and transitions. 

Electrical engineering faculty

Michael Isaacson
Developing biomimetic systems, implantable electronic devices that interact with living tissues, to restore abilities that have been lost through injury or disease.

Chemistry & biochemistry faculty

Carrie Partch
Dr. Partch is interested in the intersection between stem cells and circadian rhythms. Several years ago, her lab discovered a cancer-testis antigen, PASD1, whose expression is normally restricted to germline spermatagonial stem cells (SSCs), but becomes upregulated in a variety of somatic tissues in cancer. Circadian rhythms place daily limits on cellular proliferation, and as a consequence, circadian clocks are absent from highly proliferative SSCs and are also suppressed or lost altogether in many forms of cancer. Partch's lab recently published that PASD1 works to directly suppress clock function in cancer cells. We are now working to understand the basis by which this stem cell-specific factor suppresses the clock and regulates proliferation in cancer and stem cell populations.

Seth Rubin
Dr. Rubin is determining and targeting molecular mechanisms controlling stem cell and cancer cell division. His lab studies the structure and function of transcription factor complexes and their regulators that modulate cell-cycle dependent gene expression. They answer how these complexes are assembled, how they are regulated, and how their structural properties mediate their function. Projects include investigating critical oncogenes and tumor suppressors, such as Rb, E2F, Myb, and FoxM1, which play critical roles in stem cell division and renewal. Hypotheses for function and regulation are generated through structural biology approaches and tested with genetic manipulation and analysis of human embryonic stem cells. 

Microbiology and Environmental Toxicology faculty

Raquel Chamorro-Garcia
The Chamorro-Garcia lab is interested in better understanding mechanisms of genome-environment interactions. Environmental factors such as pollutants, diets, temperature or stress, can contribute to disease not only in individuals directly exposed to the stressor but also in their unexposed descendants. Our work integrates epigenomic, transcriptomic, and physiological analyses to reveal how environmental stressors lead to the modulation of the expression of the genome. Our current emphasis is on alterations of chromatin organization during early embryonic development, and on how this disruption is propagated through development and across generations contributing to phenotypic variation and disease. 

Ethical and societal research

Ethical and societal issues related to stem cell research constitute an important avenue of inquiry at UCSC.

Jenny Reardon, Sociology